■ 基本信息
啟動(dòng)子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal,hGH poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳系列質(zhì)粒；哺乳表達質(zhì)粒；pCMV系列質(zhì)粒 |
質(zhì)粒大小: | 6299bp |
質(zhì)粒標簽: | N-3×FLAG |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養條件: | 37度 |
表達宿主: | 293T等哺乳細胞 |
培養條件: | 37℃，5%CO2 |
誘導方式: | 無(wú)須誘導，瞬時(shí)表達 |
5'測序引物:
| CMV-F（CGCAAATGGGCGGTAGGCGTG） |
3'測序引物: | hGH-PA-R（CCAGCTTGGTTCCCAATAGA） |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418
|
熒光標記: |
|
■ 質(zhì)粒簡(jiǎn)介
p3×FLAG-CMV-10表達載體是用于在哺乳動(dòng)物細胞中建立N-3×FLAG融合蛋白的瞬時(shí)或穩定細胞內的pCMV5表達載體的升級版。該載體在多克隆區域上游編碼三個(gè)相鄰的FLAG表位（Asp-Tyr-Lys-Xaa-Xaa-Asp）。這導致使用FLAG抗體的檢測靈敏度增加。第三個(gè)FLAG表位包括腸激酶識別序列，允許從純化的融合蛋白切割3×FLAG肽。
The p3×FLAG-CMV-10 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of N-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG? epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
p3×FLAG-CMV-10 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigenexpressing
host, such as COS cells.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1697 p3×Flag-CMV-10哺乳表達質(zhì)粒.txt
