■ 基本信息
啟動(dòng)子: | 無(wú) |
復制子: | pVS1 oriV，ori |
終止子: | NOS terminator |
質(zhì)粒大小: | 6813bp |
質(zhì)粒標簽: | C-6xHis |
原核抗性: | Kan |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 植物 |
5'測序引物: | 根據序列設計引物 |
3'測序引物: | 根據序列設計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 植物 |
質(zhì)粒用途: | 蛋白表達 |
片段類(lèi)型: |
|
片段物種: |
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原核抗性: | Kan |
真核抗性: |
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熒光標記: |
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■ 質(zhì)粒簡(jiǎn)介
The pCambia vector backbone is derived from the pPZP vectors.
The pCambia0380 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
This vector contains a range of restriction sites on either side of the pUC8 polylinker, making it suitable for advanced construction purposes with users inserting their own promoters, selection genes, reporter genes. The only functional signals between the T-DNA borders are the start and stop codons, the histidine tag, and the NOS-poly(A) signal.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1711 pCAMBIA0380植物表達質(zhì)粒.txt
